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AAK1 Antibody (N-term) Blocking Peptide

Synthetic peptide

     
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Product Information
Primary Accession Q2M2I8
Clone Names 80305101
Peptide ID 80305101
Additional Information
Gene ID 22848
Other Names AP2-associated protein kinase 1, Adaptor-associated kinase 1, AAK1, KIAA1048
Target/Specificity The synthetic peptide sequence used to generate the antibody AP7861a was selected from the N-term region of human AAK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Format Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Protein Information
Name AAK1
Synonyms KIAA1048
Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Cellular Location Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity).
Tissue Location Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain
Research Areas
Citations (0)

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Background

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.

References

Henderson,D.M., Mol. Biol. Cell 18 (7), 2698-2706 (2007)Takahashi,T., Cancer Res. 66 (24), 11932-11937 (2006)Schmid,E.M., PLoS Biol. 4 (9), E262 (2006)

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$ 80.00
Cat# BP7861a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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