|Other Names||Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85, ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7992a was selected from the N-term region of human ARHG7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||COOL1, KIAA0142, P85SPR, PAK3BP, PIXB|
|Function||Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.|
|Cellular Location||Cell junction, focal adhesion. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection, lamellipodium Note=Detected at cell adhesions. A small proportion is detected at focal adhesions|
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Provided below are standard protocols that you may find useful for product applications.
Rho GTPases play a fundamental role in numerous cellular processes triggered by extracellular stimuli that work through G protein coupled receptors. ARHG7 belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. This protein can induce membrane ruffling.
Flaiz,C., Brain Pathol. 19 (1), 27-38 (2009)Eitel,J., J. Immunol. 181 (4), 2664-2671 (2008)
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