DGKZ Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q13574 |
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Clone Names | 3041110 |
Gene ID | 8525 |
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Other Names | Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta, DGKZ, DAGK6 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP8060b was selected from the C-term region of human DGKZ . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | DGKZ (HGNC:2857) |
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Synonyms | DAGK6 |
Function | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed:9159104, PubMed:19744926, PubMed:22108654). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed:15544348, PubMed:19744926, PubMed:22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed:15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity). |
Cellular Location | Nucleus. Cytoplasm, cytosol. Cell membrane. Cell projection, lamellipodium |
Tissue Location | Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellularsignaling cascade and signal transduction.
References
Hogan, A., et al., J. Biol. Chem. 276(28):26526-26533 (2001).Topham, M.K., et al., Nature 394(6694):697-700 (1998).Ding, L., et al., Proc. Natl. Acad. Sci. U.S.A. 94(11):5519-5524 (1997).Bunting, M., et al., J. Biol. Chem. 271(17):10230-10236 (1996).
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