|Other Names||Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, PAPS synthase 1, PAPSS 1, Sulfurylase kinase 1, SK 1, SK1, Sulfate adenylyltransferase, ATP-sulfurylase, Sulfate adenylate transferase, SAT, Adenylyl-sulfate kinase, 3'-phosphoadenosine-5'-phosphosulfate synthase, APS kinase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, PAPSS1, ATPSK1, PAPSS|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8090a was selected from the N-term region of human PAPSS1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway (PubMed:9576487, PubMed:9668121, PubMed:9648242, PubMed:14747722). Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (PubMed:9576487).|
|Tissue Location||Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver Also expressed in high endothelial venules (HEV) cells and in cartilage.|
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Provided below are standard protocols that you may find useful for product applications.
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes. SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2.
Venkatachalam, K.V., IUBMB Life 55(1):1-11 (2003).Xu, Z.H., et al., Biochem. Biophys. Res. Commun. 268(2):437-444 (2000).Venkatachalam, K.V., et al., J. Biol. Chem. 273(30):19311-19320 (1998).ul Haque, M.F., et al., Nat. Genet. 20(2):157-162 (1998).Girard, J.P., et al., FASEB J. 12(7):603-612 (1998).
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