|Other Names||cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2, PRKAR2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8096a was selected from the N-term region of human PKR2 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and the cell membrane|
|Tissue Location||Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible|
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Provided below are standard protocols that you may find useful for product applications.
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKR2 is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
MacDougall, M.W., et al., J. Clin. Endocrinol. Metab. 88(5):2194-2205 (2003).Birkeli, K.A., et al., J. Biol. Chem. 278(3):1991-1997 (2003).Sun, F., et al., J. Biol. Chem. 275(19):14360-14366 (2000).Zakhary, D.R., et al., J. Biol. Chem. 275(52):41389-41395 (2000).Tasken, K., et al., Genomics 50(3):378-381 (1998).
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