|Other Names||Thymidine kinase, TK|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8100a was selected from the N-term region of Epstein-Barr thymidine kinase . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.|
|Cellular Location||Virion tegument. Host nucleus. Note=Localizes to the centrosome and more precisely to the periphery of the centriole, tightly encircling the tubulin-rich centrioles|
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Provided below are standard protocols that you may find useful for product applications.
Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis.
Littler, E., et al., EMBO J. 5(8):1959-1966 (1986).Bankier, A.T., et al., Mol. Biol. Med. 1(1):21-45 (1983).
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