|Other Names||Diacylglycerol kinase epsilon, DAG kinase epsilon, Diglyceride kinase epsilon, DGK-epsilon, DGKE, DAGK5|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8125a was selected from the N-term region of human DGKE . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.|
|Cellular Location||Membrane; Multi-pass membrane protein. Membrane Cytoplasm|
|Tissue Location||Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGKE shows specificity for arachidonyl-containing diacylglycerol. DGKE is expressed predominantly in testis.
Tang, W., et al., Gene 239(1):185-192 (1999).Hart, T.C., et al., Genomics 56(2):233-235 (1999).Tang, W., et al., J. Biol. Chem. 271(17):10237-10241 (1996).
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