DGKE Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P52429 |
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Clone Names | 3051405 |
Gene ID | 8526 |
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Other Names | Diacylglycerol kinase epsilon, DAG kinase epsilon, Diglyceride kinase epsilon, DGK-epsilon, DGKE, DAGK5 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP8125b was selected from the C-term region of human DGKE . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | DGKE |
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Synonyms | DAGK5 |
Function | Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:19744926, PubMed:22108654, PubMed:21477596, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:8626589, PubMed:15544348). Also plays an important role in the biosynthesis of complex lipids (PubMed:8626589). Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2- (5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle (PubMed:19744926, PubMed:22108654, PubMed:23274426). Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently (PubMed:22108654). |
Cellular Location | Membrane; Single- pass membrane protein. Cytoplasm |
Tissue Location | Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGKE shows specificity for arachidonyl-containing diacylglycerol. DGKE is expressed predominantly in testis.
References
Tang, W., et al., Gene 239(1):185-192 (1999).Hart, T.C., et al., Genomics 56(2):233-235 (1999).Tang, W., et al., J. Biol. Chem. 271(17):10237-10241 (1996).
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