|Other Names||Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8400a was selected from the region of human Phospho-Mlf1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.|
|Cellular Location||Cytoplasm. Nucleus. Note=In non- hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM|
|Tissue Location||Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
Arber, D.A., et al., Hum. Pathol. 34(8):809-813 (2003).Lim, R., et al., J. Biol. Chem. 277(43):40997-41008 (2002).Yoneda-Kato, N., et al., Oncogene 12(2):265-275 (1996).
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