|Other Names||Dual specificity protein phosphatase 3, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, VHR, DUSP3, VHR|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8446a was selected from the C-term region of human DUSP3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.|
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Provided below are standard protocols that you may find useful for product applications.
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. The gene for this protein maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer.
Alonso, A., et al., Nat. Immunol. 4(1):44-48 (2003).Alonso, A., et al., J. Biol. Chem. 276(7):4766-4771 (2001).Todd, J.L., et al., J. Biol. Chem. 274(19):13271-13280 (1999).Kamb, A., et al., Genomics 23(1):163-167 (1994).Folander, K., et al., Genomics 23(1):295-296 (1994).
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