|Other Names||Dual specificity protein phosphatase 4, Dual specificity protein phosphatase hVH2, Mitogen-activated protein kinase phosphatase 2, MAP kinase phosphatase 2, MKP-2, DUSP4, MKP2, VH2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8447a was selected from the Center region of human DUSP4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.|
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Provided below are standard protocols that you may find useful for product applications.
DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus.
Chen, P., et al., J. Biol. Chem. 276(31):29440-29449 (2001).Smith, A., et al., Genomics 42(3):524-527 (1997).Chu, Y., et al., J. Biol. Chem. 271(11):6497-6501 (1996).King, A.G., et al., Oncogene 11(12):2553-2563 (1995).Guan, K.L., et al., J. Biol. Chem. 270(13):7197-7203 (1995).
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