|Other Names||Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform, PPP3CA, CALNA, CNA|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8463b was selected from the C-term region of human PPP3CA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.|
|Cellular Location||Cell membrane. Cell membrane, sarcolemma. Nucleus. Note=Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
PPP3CA is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.
Yoshizaki, T., et al., J. Biol. Chem. 279(21):22715-22726 (2004).Flajolet, M., et al., Proc. Natl. Acad. Sci. U.S.A. 100(26):16006-16011 (2003).Ofek, P., et al., J. Biol. Chem. 278(16):14299-14305 (2003).Matsuda, A., et al., Oncogene 22(21):3307-3318 (2003).Cheng, A., et al., Genes Dev. 13(22):2946-2957 (1999).
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