|Other Names||Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, PP2C-alpha, Protein phosphatase IA, PPM1A, PPPM1A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8466b was selected from the C-term region of human PPM1A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.|
|Cellular Location||Nucleus. Cytoplasm, cytosol. Membrane. Note=Weakly associates at the membrane and N- myristoylation mediates the membrane localization.|
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PPM1A is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.
Yoshizaki, T., et al., J. Biol. Chem. 279(21):22715-22726 (2004).Flajolet, M., et al., Proc. Natl. Acad. Sci. U.S.A. 100(26):16006-16011 (2003).Ofek, P., et al., J. Biol. Chem. 278(16):14299-14305 (2003).Matsuda, A., et al., Oncogene 22(21):3307-3318 (2003).Cheng, A., et al., Genes Dev. 13(22):2946-2957 (1999).
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