|Other Names||Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, TBP-interacting protein of 120 kDa A, TBP-interacting protein 120A, p120 CAND1, CAND1, KIAA0829, TIP120, TIP120A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8519c was selected from the Center region of human TIP120A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA0829, TIP120, TIP120A|
|Function||Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.|
|Cellular Location||Cytoplasm. Nucleus. Note=Predominantly cytoplasmic|
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Provided below are standard protocols that you may find useful for product applications.
TIP120A enhances transcription from various types of promoters. It is regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. It prevents neddylation of CUL1 by physically blocking access to the neddylation site and disrupts interactions between CUL1 and SKP1A and between CUL1 and F-box proteins.
Feng,S., et.al., Plant Cell 16 (7), 1870-1882 (2004)Zheng,J., et.al., Mol. Cell 10 (6), 1519-1526 (2002)
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