EIF3S2 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q13347 |
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Clone Names | 81202031 |
Gene ID | 8668 |
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Other Names | Eukaryotic translation initiation factor 3 subunit I {ECO:0000255|HAMAP-Rule:MF_03008}, eIF3i {ECO:0000255|HAMAP-Rule:MF_03008}, Eukaryotic translation initiation factor 3 subunit 2 {ECO:0000255|HAMAP-Rule:MF_03008}, TGF-beta receptor-interacting protein 1, TRIP-1, eIF-3-beta {ECO:0000255|HAMAP-Rule:MF_03008}, eIF3 p36 {ECO:0000255|HAMAP-Rule:MF_03008}, EIF3I {ECO:0000255|HAMAP-Rule:MF_03008} |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP8547a was selected from the N-term region of human EIF3S2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | EIF3I {ECO:0000255|HAMAP-Rule:MF_03008} |
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Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl- tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). |
Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03008}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
EIF3S2 is the largest of the EIFs. It consists of at least 10 nonidentical subunits in mammals. In S. cerevisiae the p39 subunit contains WD repeats; these are thought to mediate protein-protein interactions. The p39 protein appears to be essential for maintaining the integrity of the yeast EIF3 complex. The mammalian EIF3-p36 subunit is homologous to yeast p39.
References
Navarro,A.,et.al., Am. J. Physiol. Lung Cell Mol. Physiol. 296 (6), L928-L935 (2009)Perard,J.,et.al.,FEBS Lett. 583 (1), 70-74 (2009)
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