|Other Names||E3 ubiquitin-protein ligase RNF5, 632-, Protein G16, RING finger protein 5, Ram1 homolog, HsRma1, RNF5, G16, NG2, RMA1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8608a was selected from the N-term region of human RNF5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||G16, NG2, RMA1|
|Function||Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.|
|Cellular Location||Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
RNF5 contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.
Didier,C., et.al., Mol. Cell. Biol. 23 (15), 5331-5345 (2003)Bromberg,K.D., et.al., Cancer Res. 67 (17), 8172-8179 (2007)
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