|Other Names||Growth factor receptor-bound protein 14, GRB14 adapter protein, GRB14|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8969a was selected from the N-term region of human GRB14. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.|
|Cellular Location||Cytoplasm. Endosome membrane; Peripheral membrane protein. Note=Upon insulin stimulation, translocates to the plasma membrane.|
|Tissue Location||Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
GRB14 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling.
Jones,N., et.al., J. Biol. Chem. 274 (43), 30896-30905 (1999)Reilly,J.F., et.al., J. Biol. Chem. 275 (11), 7771-7778 (2000)
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