|Other Names||Hydroxyacid-oxoacid transhydrogenase, mitochondrial, HOT, Alcohol dehydrogenase iron-containing protein 1, ADHFe1, Fe-containing alcohol dehydrogenase, ADHFE1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP9062c was selected from the Center region of human ADHFE1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2- hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3- hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.|
|Tissue Location||Only expressed in adult liver.|
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Provided below are standard protocols that you may find useful for product applications.
The ADHFE1 protein encodes hydroxyacid-oxoacid transhydrogenase (EC 220.127.116.11), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues.
Lyon,R.C., et.al., Chem. Biol. Interact. 178 (1-3), 283-287 (2009)Kardon,T., et.al., FEBS Lett. 580 (9), 2347-2350 (2006)
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