|Other Names||Carbohydrate sulfotransferase 7, 282-, Chondroitin 6-sulfotransferase 2, C6ST-2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5, GST-5, N-acetylglucosamine 6-O-sulfotransferase 4, GlcNAc6ST-4, Gn6st-4, CHST7|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP9158c was selected from the Center region of human CHST7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N- acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc.|
|Cellular Location||Golgi apparatus membrane; Single-pass type II membrane protein|
|Tissue Location||Widely expressed. Highly expressed in heart, spleen, liver and ovary. Expressed at lower level in brain, placenta, thyroid, spinal cord and peripheral blood leukocytes Not expressed in adult skin.|
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Provided below are standard protocols that you may find useful for product applications.
CHST7 catalyzes the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. It preferentially acts on mannose-linked GlcNAc and also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. It also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. This protein has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. It catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha-or beta-benzyl GalNAc.
Liu T., et.al., J. Proteome Res. 4:2070-2080(2005).
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