|Other Names||F-box only protein 3, FBXO3, FBX3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP9195b was selected from the C-term region of human FBXO3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Substrate recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53- dependent transactivation.|
|Cellular Location||Nucleus. Note=Colocalizes with PML at the peripheries of nuclear bodies|
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Provided below are standard protocols that you may find useful for product applications.
FBXO3 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs.
Shima,Y., et.al., Mol. Cell. Biol. 28 (23), 7126-7138 (2008)Ilyin,G.P., et.al., Genomics 67 (1), 40-47 (2000)
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