FBXL5 Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9UKA1 |
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Gene ID | 26234 |
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Other Names | F-box/LRR-repeat protein 5, F-box and leucine-rich repeat protein 5, F-box protein FBL4/FBL5, p45SKP2-like protein, FBXL5, FBL4, FBL5, FLR1 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | FBXL5 |
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Synonyms | FBL4, FBL5, FLR1 |
Function | Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2 (PubMed:19762596, PubMed:19762597). The C-terminal domain of FBXL5 contains a redox-sensitive [2Fe-2S] cluster that, upon oxidation, promotes binding to IRP2 to effect its oxygen-dependent degradation (PubMed:32126207). Under iron deficiency conditions, the N-terminal hemerythrin-like (Hr) region, which contains a diiron metal center, cannot bind iron and undergoes conformational changes that destabilize the FBXL5 protein and cause its ubiquitination and degradation (PubMed:19762596, PubMed:19762597). When intracellular iron levels start rising, the Hr region is stabilized (PubMed:19762596, PubMed:19762597). Additional increases in iron levels facilitate the assembly and incorporation of a redox active [2Fe-2S] cluster in the C- terminal domain (PubMed:32126207). Only when oxygen level is high enough to maintain the cluster in its oxidized state can FBXL5 recruit IRP2 as a substrate for polyubiquination and degradation (PubMed:32126207). Promotes ubiquitination and subsequent degradation of the dynactin complex component DCTN1 (PubMed:17532294). Within the nucleus, promotes the ubiquitination of SNAI1; preventing its interaction with DNA and promoting its degradation (PubMed:24157836). Negatively regulates DNA damage response by mediating the ubiquitin- proteasome degradation of the DNA repair protein NABP2 (PubMed:25249620). |
Cellular Location | Cytoplasm, perinuclear region. Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
References
Salahudeen, A.A., et al. Science 326(5953):722-726(2009)Vashisht, A.A., et al. Science 326(5953):718-721(2009)Zhang, N., et al. Biochem. Biophys. Res. Commun. 359(1):34-39(2007)Ilyin, G.P., et al. Genomics 67(1):40-47(2000)Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)Cenciarelli, C., et al. Curr. Biol. 9(20):1177-1179(1999)
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