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CDK8 Over-expression LysateGene specific transient over-expression cell lysates
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| LY10109a | 100ug 400 ul | 3-4 weeks | $ 325.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CDK8 Over-expression Lysate - Product info | |
| Application | WB
|
| Other Accession | NM_001260.1 |
CDK8 Over-expression Lysate - Additional info | |
| Gene Symbol | CDK8 |
| Other Names K35, MGC126074, MGC126075 | |
| Format over-expression cell lysate in RIPA buffer | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. | |
| Precautions This product is for research use only. Not for use in diagnostic or therapeutic procedures. | |
CDK8 Over-expression Lysate - Related products
AP11972c: CDK8 Antibody (Center)
AP7524a: CDK8 Antibody (N-term)
AP7524d: CDK8 Antibody (N-term)
CDK8 Over-expression Lysate - Application data
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Western blot analysis of his-tag CDK8 (arrow) using his tag antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK8 gene (Lane 2, Cat. #LY10109a).
CDK8 Over-expression Lysate - Research Areas
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BACKGROUND
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
REFERENCES
[Mechanisms of transcriptional regulation upon protein phosphorylation by mediator complexes] Tsutsui T, et al. Seikagaku, 2010 Mar. PMID 20408451. CDK8 is a positive regulator of transcriptional elongation within the serum response network. Donner AJ, et al. Nat Struct Mol Biol, 2010 Feb. PMID 20098423. Genome-wide analysis of chromosomal alterations in patients with esophageal squamous cell carcinoma exposed to tobacco and betel quid from high-risk area in India. Chattopadhyay I, et al. Mutat Res, 2010 Feb 2. PMID 20083228. Nuclear CDKs drive Smad transcriptional activation and turnover in BMP and TGF-beta pathways. Alarc?n C, et al. Cell, 2009 Nov 13. PMID 19914168. CDK8 expression in 470 colorectal cancers in relation to beta-catenin activation, other molecular alterations and patient survival. Firestein R, et al. Int J Cancer, 2010 Jun 15. PMID 19790197.
