MAP2K2 Over-expression LysateGene specific transient over-expression cell lysates

BACKGROUND

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.

REFERENCES

Molecular and functional analysis of a novel MEK2 mutation in cardio-facio-cutaneous syndrome: transmission through four generations. Rauen KA, et al. Am J Med Genet A, 2010 Apr. PMID 20358587. Investigation of gene dosage imbalances in patients with Noonan syndrome using multiplex ligation-dependent probe amplification analysis. Nystr?m AM, et al. Eur J Med Genet, 2010 May-Jun. PMID 20302979. Distinct effects of knocking down MEK1 and MEK2 on replication of herpes simplex virus type 2. Zhang H, et al. Virus Res, 2010 Jun. PMID 20172001. Helicobacter pylori lipopolysaccharides upregulate toll-like receptor 4 expression and proliferation of gastric epithelial cells via the MEK1/2-ERK1/2 mitogen-activated protein kinase pathway. Yokota S, et al. Infect Immun, 2010 Jan. PMID 19858308. RNAi-mediated MEK1 knock-down prevents ERK1/2 activation and abolishes human hepatocarcinoma growth in vitro and in vivo. Gailhouste L, et al. Int J Cancer, 2010 Mar 15. PMID 19816936.