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VRK3 Over-expression LysateGene specific transient over-expression cell lysates
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| LY10319a | 100ug 400 ul | 3-4 weeks | $ 325.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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VRK3 Over-expression Lysate - Product info | |
| Application | WB
|
| Other Accession | NM_001025778.1 |
VRK3 Over-expression Lysate - Additional info | |
| Gene Symbol | VRK3 |
| Format over-expression cell lysate in RIPA buffer | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. | |
| Precautions This product is for research use only. Not for use in diagnostic or therapeutic procedures. | |
VRK3 Over-expression Lysate - Related products
AP7410c: VRK3 Antibody (Center)
VRK3 Over-expression Lysate - Application data
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Western blot analysis of his-tag VRK3 (arrow) using his tag antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VRK3 gene (Lane 2, Cat. #LY10319a).
VRK3 Over-expression Lysate - Research Areas
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BACKGROUND
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms.
REFERENCES
Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Sanz-Garc?a M, et al. Mol Cell Proteomics, 2008 Nov. PMID 18617507. Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334. Characterization of three paralogous members of the Mammalian vaccinia related kinase family. Nichols RJ, et al. J Biol Chem, 2004 Feb 27. PMID 14645249. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.
