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Recombinant Human FGF-acidic

Product Information
Additional Information
Description FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 16.0 kDa protein consisting of 141 amino acid residues.
BiologicalActivity Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.

Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence of 10 μg/ml heparin, corresponding to a specific activity of ≥ 2 x 106 units/mg.
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin Endotoxin level is <0.1 ng/ µg of protein (<1EU/ µg).
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
PrecautionsRecombinant Human FGF-acidic is for research use only and not for use in diagnostic or therapeutic procedures.
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$ 216.00
$ 96.00
Cat# PBG10105
(40 western blots)
Availability: 5-7days
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