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Recombinant Human GASP-1

Product Information
Additional Information
Description Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-β binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66 kDa by SDS-PAGE analysis under non-reducing conditions.
BiologicalActivity Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells.  The ED50 for this effect is 0.0025-0.0040 µg/ml in the presence of 5ng/ml of human Myostatin (GDF-8).
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin Endotoxin level is <0.1 ng/ µg of protein (<1EU/ µg).
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
PrecautionsRecombinant Human GASP-1 is for research use only and not for use in diagnostic or therapeutic procedures.
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$ 216.00
$ 96.00
Cat# PBG10126
(40 western blots)
Availability: 5-7days
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