Recombinant Rat G-CSF
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Recombinant rat G-CSF is a 21.6 kDa protein consisting of 196 amino acid residues. |
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BiologicalActivity | Determined by its ability to stimulate the proliferation of M NFS-60 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg. |
Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
Endotoxin | Endotoxin level is <0.1 ng/ µg of protein (<1EU/ µg). |
Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
Storage | -20°C |
Precautions | Recombinant Rat G-CSF is for research use only and not for use in diagnostic or therapeutic procedures. |
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