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Recombinant Murine Granzyme B

Product Information
Additional Information
Description Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry.
BiologicalActivity Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 250C, is greater than 750 nM/min per μg of enzyme.
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin Endotoxin level is <0.1 ng/ µg of protein (<1EU/ µg).
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
PrecautionsRecombinant Murine Granzyme B is for research use only and not for use in diagnostic or therapeutic procedures.
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$ 216.00
$ 96.00
Cat# PBG10147
(40 western blots)
Availability: 5-7days
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