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Recombinant Human IFN-β

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Additional Information
Description Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNα is an acid stable molecule that signals through IFNα/βR, which is also used by IFNβ. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNα contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Recombinant human IFNβ is an 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFNβ has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.
BiologicalActivity Assay #1:Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml.

Assay #2: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin Endotoxin level is <0.1 ng/ µg of protein (<1EU/ µg).
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
PrecautionsRecombinant Human IFN-β is for research use only and not for use in diagnostic or therapeutic procedures.
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$ 216.00
$ 96.00
Cat# PBG10162
(40 western blots)
Availability: 5-7days
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