|Calculated MW||55357 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Caspase-8. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Caspase-8, CASP-8, 18.104.22.168, Caspase-8 subunit p18, Caspase-8 subunit p10, Casp8|
|Formulation||50 µg (0.2 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 0.1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Caspase-8 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.|
|Tissue Location||Expressed in a wide variety of tissues. Highest expression in spleen, thymus, lung, liver and kidney Lower expression in heart, brain, testis and skeletal muscle|
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