|Calculated MW||11743 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Cytochrome c. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30 minutes at 37oC.|
|Other Names||Cytochrome c, testis-specific, Cyct|
|Formulation||50 µg (0.2 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 0.1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cytochrome c Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.|
|Cellular Location||Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane|
|Tissue Location||This is one of two isocytochromes C found in the testis. The other is identical with the form found in other rat tissues. These cytochromes are assumed to be located in the sperm|
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