|Calculated MW||49736 Da|
|Other Names||Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TY|
|Application Notes||Reconstitute to 1 unit per µl in PBS containing 15% glycerol.|
|Storage||-70°C; Lyophilized powder|
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Provided below are standard protocols that you may find useful for product applications.
Caspase-5 (also know as ICErelIII, TY) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-5 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-5 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-5 activation and roles of caspase-5 in apoptosis are poorly understood. The recombinant active human caspase-5 was expressed in E. coli. The active caspase-5 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA or Ac-WEHD-AFC
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