Caspase-9, human recombinant protein
Caspase-9
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P55211 |
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Calculated MW | 46281 Da |
Gene ID | 842 |
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Gene Symbol | CASP9 |
Other Names | Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, LEHDase |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Caspase-9 |
Application Notes | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
Format | Lyophilized powder |
Storage | -70°C; Semi Dry powder |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-9 is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger activation of downstream effector caspases, leading to disassembly of cell structures. The recombinant active human caspase-9 was expressed in E. coli. The active caspase-9 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-LEHD-pNA or Ac-LEHD-AFC
References
Duan H.,et al.J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M.,et al.J. Biol. Chem. 271:27099-27106(1996).
Hadano S.,et al.Mamm. Genome 10:757-760(1999).
Srinivasula S.M.,et al.Cancer Res. 59:999-1002(1999).
Izawa M.,et al.Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
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