Caspase-9 Proform, human recombinant protein
Caspase-9 Proform
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Concentration | 2 |
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Other Names | Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, LEHDase |
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Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Caspase-9 |
Format | Liquid |
Storage | -70°C; 2 µg/ µl in 1 mM acidic acid |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-9 is a member of the caspase family of cysteine proteases. Caspase-9 exists in cells as an inactive proenzyme. Proteolytic processing of this inactive proenzyme generates the large and small subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be the key signal-transducing events in apoptosis. Caspase-9 is considered to be an upstream enzyme that initiates caspase cascade leading to apoptosis. The recombinant Procaspase-9 is produced by expression of a human cDNA in E. coli and further purified by multiple chromatographic steps.
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