|Calculated MW||22893 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Hsp27. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C|
|Other Names||Heat shock protein beta-1, HspB1, Heat shock 27 kDa protein, HSP 27, Hspb1, Hsp27|
|Formulation||50 µg (0.2 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Hsp27 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in stress resistance and actin organization.|
|Cellular Location||Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity).|
|Tissue Location||Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles|
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