|Other Names||SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog|
|Target/Specificity||Sonic Hedgehog (SHH)|
|Application Notes||When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.|
|Storage||-20°C; Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Recombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.
Marigo V.,et al.Genomics 28:44-51(1995).
Tate G.,et al.J. Biochem. Mol. Biol. Biophys. 4:27-34(2000).
Hillier L.W.,et al.Nature 424:157-164(2003).
Scherer S.W.,et al.Science 300:767-772(2003).
Pepinsky R.B.,et al.J. Biol. Chem. 273:14037-14045(1998).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.