|Calculated MW||19.8 kDa|
|Other Names||SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog|
|Results||0.03 – 0.30 ug/ml.|
|Target/Specificity||Sonic Hedgehog (SHH)|
|Application Notes||When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.|
|Storage||-20°C; Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.|
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Provided below are standard protocols that you may find useful for product applications.
Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da.
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