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FGF-2/FGF-basic, rat recombinant protein
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast Growth Factor
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P13109 |
|---|---|
| Calculated MW | 16.3 kDa |
| Gene ID | 54250 |
|---|---|
| Gene Symbol | FGF-2 |
| Other Names | FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2 |
| Gene Source | Rat |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥97% |
| Assay2&Purity2 | HPLC; ≥97% |
| Recombinant | Yes |
| Results | <0.2 ng/ml |
| Target/Specificity | FGF-2 |
| Application Notes | Reconstitute in sterile H₂O to 0.1–1 mg /ml. The solution can then further diluted to other aqueous solutions |
| Format | Lyophilized protein |
| Storage | -20°C; Lyophilized from 1 mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130 mM NaCl. |
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Provided below are standard protocols that you may find useful for product applications.
Background
FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. The recombinant rat FGF-2 is a single, non-glycosylated chain containing 145 amino acids and having a molecular weight of 16.3 kDa.
References
Shimasaki S.,et al.Biochem. Biophys. Res. Commun. 157:256-263(1988).
Kurokawa T.,et al.Nucleic Acids Res. 16:5201-5201(1988).
Pasumarthi K.B.S.,et al.J. Neurochem. 68:898-908(1997).
El-Husseini A.E.-D.,et al.Biochim. Biophys. Acta 1131:314-316(1992).
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