|Calculated MW||17.25 kDa|
|Other Names||FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2|
|Application Notes||Reconstitute in sterile water to a concentration not less than 0.1 mg/ml. Aliquot and store at –20°C. Avoid freeze-thaw cycles.|
|Storage||-20°C; Sterile filtered white lyophilized powder. Prepared fom a sterile solution containing 1 % HSA.|
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Provided below are standard protocols that you may find useful for product applications.
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. FGF-2 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
Abraham J.A.,et al.Science 233:545-548(1986).
Abraham J.A.,et al.Cold Spring Harb. Symp. Quant. Biol. 51:657-668(1986).
Esch F.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6507-6511(1985).
Ueno N.,et al.Biochem. Biophys. Res. Commun. 138:580-588(1986).
Gospodarowicz D.,et al.Endocrinology 118:82-90(1986).
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