|Calculated MW||29274 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of CD40L. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C|
|Other Names||CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||CD40L Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CD40L, TNFSF5, TRAP|
|Function||Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T- cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein Cell surface|
|Tissue Location||Specifically expressed on activated CD4+ T- lymphocytes|
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