|Calculated MW||53322 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of RIP3. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Receptor-interacting serine/threonine-protein kinase 3, 220.127.116.11, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, mRIP3, Ripk3, Rip3|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 0.1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||RIP3 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.|
|Cellular Location||Cytoplasm, cytosol. Cell membrane. Mitochondrion|
|Tissue Location||Expressed in embryo and in adult spleen, liver, testis, heart, brain and lung|
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