|Calculated MW||26132 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Bcl-xL. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 0.1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Bcl-xL Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage- dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.|
|Cellular Location||Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to the centrosome when phosphorylated at Ser-49 Isoform Bcl-X(delta-TM): Cytoplasm.|
|Tissue Location||Widely expressed, with highest levels in the brain, thymus, bone marrow, and kidney. Bcl-X(L) and Bcl-X(delta- TM) expression is enhanced in B- and T-lymphocytes that have been activated.|
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