IL-12, human recombinant protein
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-α,
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P29459 |
---|---|
Calculated MW | 75.0 kDa |
Gene ID | 3592 |
---|---|
Gene Symbol | IL12 |
Other Names | NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-α, IL-12 |
Gene Source | Human |
Source | CHO cells |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Results | <0.1 ng/ml, |
Target/Specificity | IL-12 |
Application Notes | Reconstitute in 1X PBS (pH 7.2-7.4) to a concentration of 0.2 mg/ml. The solution can then diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. |
Format | Lyophilized protein |
Storage | -20°C; Sterile filtered and lyophilized in 0.5X PBS, pH 7.2 |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
IL-12 is a potent regulator of cell mediated immune responses and in induces IFN- production by NK and T cells. It is produced by activated monocytes/macrophage cells, B-lymphocytes, and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+, and CD8+ cells and induce the development of IFN- producing Th1 cells. Recombinant human IL-12 is a 75 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
References
Wolf S.F.,et al.J. Immunol. 146:3074-3081(1991).
Gubler U.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:4143-4147(1991).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Stern A.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:6808-6812(1990).
Merberg D.M.,et al.Immunol. Today 13:77-78(1992).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.