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ILK1 Blocking Peptide

     
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Product Information
Primary Accession O55222
Other Accession NP_034692
Gene ID 16202
Calculated MW 51373 Da
Additional Information
Gene ID 16202
Application & Usage The peptide is used for blocking the antibody activity of ILK1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Other Names Integrin-linked protein kinase, 2.7.11.1, Ilk
Target/Specificity ILK1
Formulation 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsILK1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Ilk {ECO:0000312|MGI:MGI:1195267}
Function Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Regulates cell motility by forming a complex with PARVB. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side {ECO:0000250|UniProtKB:Q13418}. Cytoplasm, myofibril, sarcomere {ECO:0000250|UniProtKB:Q13418}. Cell projection, lamellipodium
Tissue Location Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis
Research Areas
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Discontinued
Cat# PBV10180b-50
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