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Fbx32/MAFbx/ATR-1 Blocking Peptide

Product Information
Primary Accession Q91Z62
Gene ID 171043
Additional Information
Application & Usage The peptide is used for blocking the antibody activity of Fbx32/MAFbx/AT-1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Other Names F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein, MAFbx, Fbxo32
Target/Specificity Fbx32
Formulation 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsFbx32/MAFbx/ATR-1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Fbxo32
Function Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1 (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q969P5}. Nucleus {ECO:0000250|UniProtKB:Q969P5}. Note=Shuttles between cytoplasm and the nucleus. {ECO:0000250|UniProtKB:Q969P5}
Tissue Location Specifically expressed in cardiac and skeletal muscle EMBL; AY059628; AAL16406.1; -; mRNA RefSeq; NP_598205.1; NM_133521.1 UniGene; Rn.72619; - ProteinModelPortal; Q91Z62; - SMR; Q91Z62; - STRING; 10116.ENSRNOP00000010361; - PaxDb; Q91Z62; - GeneID; 171043; - KEGG; rno:171043; - UCSC; RGD:620373; rat CTD; 114907; - RGD; 620373; Fbxo32 eggNOG; KOG3926; Eukaryota eggNOG; ENOG410YPWK; LUCA HOGENOM; HOG000263478; - HOVERGEN; HBG051572; - InParanoid; Q91Z62; - KO; K10305; - UniPathway; UPA00143; - PRO; PR:Q91Z62; - Proteomes; UP000002494; Unplaced GO; GO:0005737; C:cytoplasm; ISS:UniProtKB GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell GO; GO:0000151; C:ubiquitin ligase complex; NAS:BHF-UCL GO; GO:0004842; F:ubiquitin-protein transferase activity; TAS:RGD GO; GO:0014889; P:muscle atrophy; IDA:RGD GO; GO:0010614; P:negative regulation of cardiac muscle hypertrophy; IMP:BHF-UCL GO; GO:0010666; P:positive regulation of cardiac muscle cell apoptotic process; IGI:BHF-UCL GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB GO; GO:0014894; P:response to denervation involved in regulation of muscle adaptation; ISS:UniProtKB GO; GO:0014878; P:response to electrical stimulus involved in regulation of muscle adaptation; IDA:UniProtKB GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:RGD InterPro; IPR036047; F-box-like_dom_sf SUPFAM; SSF81383; SSF81383; 1 2: Evidence at transcript level; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Ubl conjugation pathway CHAIN 1 350 F-box only protein 32 /FTId=PRO_0000119924 DOMAIN 218 266 F-box MOTIF 57 62 Nuclear localization signal MOTIF 164 168 Nuclear export signal. MOTIF 275 290 Bipartite nuclear localization signal SEQUENCE 350 AA; 40926 MW; 24AB59BC74F72472 CRC64; MPFLGQDWRS PGQSWVKTAD GWKRFLDEKS GTFVSDLSSY CNKENLFNSL NYDVAAKKRK KDIQNSKTKT QYFHQEKWIY VHKGSTKERH GYCTLGEAFN RLDFSTAILD SRRFNYVVRL LELIAKSQLT SLSGIAQKNF MNILEKVVLK VLEDQQNIRL IRELLQTLYT SLCTLVQRVG KSVLVGNINM WVYRMETTLH WQQQLNSIQI SRPAFKGLTI TDLPVCLQLN IMQRLSDGRD LVSLGQAAPD LHVLSEDRLL WKRLCQYHFS ERQIRKRLIL SDKGQLDWKK MYFKLVRCYP RREQYGVTLQ LCKHCHILSW KGTDHPCTAN NPESCSVSLS PQDFINLFKF
Research Areas
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$ 95.00
Cat# PBV10221b-50
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