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Apo-D, human recombinant protein
Apolipoprotein D, Apo-D, ApoD
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P05089 |
|---|---|
| Calculated MW | 19.8 kDa |
| Gene ID | 347 |
|---|---|
| Gene Symbol | Apo-D |
| Other Names | Apolipoprotein D, Apo-D, ApoD, Apolipoprotein, apolipoproteins, |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | HPLC; ≥95% |
| Recombinant | Yes |
| Target/Specificity | ApoD |
| Application Notes | Reconstitute in dH₂O to a working volume of 0.5 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. |
| Format | Lyophilized protein |
| Storage | -20°C; Lyophilized from 1 mg/ml in 4 mM KH2PO4, 16 mM Na2HPO4 and 115 mM NaCl pH7.5. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Apolipoprotein-D (Apo-D) is mainly associated with high density lipoproteins in human plasma. It is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apo-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue. The recombinant human Apo-D expressed from E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa.
References
Haraguchi Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:412-415(1987).
Takiguchi M.,et al.Nucleic Acids Res. 16:8789-8802(1988).
Lee Y.T.,et al.Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).
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