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G3BP Blocking Peptide

Product Information
Primary Accession Q13283
Gene ID 10146
Calculated MW 52164 Da
Additional Information
Gene ID 10146
Application & Usage The peptide is used for blocking the antibody activity of G3BP. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Other Names Ras GTPase-activating protein-binding protein 1, G3BP-1,,, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP
Target/Specificity G3BP
Formulation 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsG3BP Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name G3BP1
Synonyms G3BP
Function ATP- and magnesium-dependent helicase (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Phosphorylation-dependent sequence-specific endoribonuclease in vitro (PubMed:11604510). Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510). May be a regulated effector of stress granule assembly (PubMed:12642610, PubMed:20180778).
Cellular Location Cytoplasm, cytosol. Perikaryon {ECO:0000250|UniProtKB:P97855}. Cytoplasm, Stress granule. Nucleus. Note=Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778) The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610)
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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$ 95.00
Cat# PBV10268b-50
Availability: 5-7days
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