Leptin Receptor, human recombinant protein
OB Protein, Obesity Protein, OBS, Obesity factor
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P41159 |
---|---|
Calculated MW | 95.0 kDa (predicted) 130-140 kDa on a gel. |
Gene ID | 3952 |
---|---|
Gene Symbol | LEP |
Other Names | OB Protein, Obesity Protein, OBS, Obesity factor, HuB219, OB receptor, CD_antigen=CD295 |
Gene Source | Human |
Source | Human |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | A DNA sequence encoding the extracellular domain (Met 1 - Asp 839) of human leptin receptor (NP_002294.2) was expressed and fused with a C-terminal polyhistidine tag. Predicted NT sequence is Phe 22. |
Target/Specificity | Leptin Receptor |
Application Notes | Centrifuge the vial prior to opening. The lyophilized Leptin Binding Domain is very soluble in H₂O and most aqueous buffers below and above the isoelectric point (~ 5.9). |
Format | Lyophilized protein |
Storage | -70°C; Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Leptin receptor (LEPR), also known as OB-R and B219, is a single transmembrane-domain receptor of the cytokine receptor family. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is expressed at high levels in hematopoietic stem cells, lymphohematopoietic cell lines, as well as adult reproductive organs. Several isoforms of LEPR have been identified, and LEPR structurally contains two hemopoietin receptor domains, a fibronectin type III domain and a WSXWS domain within the extracellular region. Interaction of leptin and leptin receptor is crucial for bodyweight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.
References
Zhang Y.,et al.Nature 372:425-432(1994).
Zhang Y.,et al.Nature 374:479-479(1995).
Masuzaki H.,et al.Diabetes 44:855-858(1995).
Gong D.W.,et al.J. Biol. Chem. 271:3971-3974(1996).
Chehab F.F.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.