|Calculated MW||95.0 kDa (predicted) 130-140 kDa on a gel.|
|Other Names||OB Protein, Obesity Protein, OBS, Obesity factor, HuB219, OB receptor, CD_antigen=CD295|
|Sequence||A DNA sequence encoding the extracellular domain (Met 1 - Asp 839) of human leptin receptor (NP_002294.2) was expressed and fused with a C-terminal polyhistidine tag. Predicted NT sequence is Phe 22.|
|Application Notes||Centrifuge the vial prior to opening. The lyophilized Leptin Binding Domain is very soluble in H₂O and most aqueous buffers below and above the isoelectric point (~ 5.9).|
|Storage||-70°C; Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.|
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Provided below are standard protocols that you may find useful for product applications.
Leptin receptor (LEPR), also known as OB-R and B219, is a single transmembrane-domain receptor of the cytokine receptor family. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is expressed at high levels in hematopoietic stem cells, lymphohematopoietic cell lines, as well as adult reproductive organs. Several isoforms of LEPR have been identified, and LEPR structurally contains two hemopoietin receptor domains, a fibronectin type III domain and a WSXWS domain within the extracellular region. Interaction of leptin and leptin receptor is crucial for bodyweight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.
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Gong D.W.,et al.J. Biol. Chem. 271:3971-3974(1996).
Chehab F.F.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
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