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VASP Blocking Peptide

Product Information
Primary Accession P50552
Gene ID 7408
Calculated MW 39830 Da
Additional Information
Gene ID 7408
Application & Usage The peptide is used for blocking the antibody activity of VASP. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Other Names Vasodilator-stimulated phosphoprotein, VASP, VASP
Target/Specificity VASP
Formulation 50 µg (0.5 mg/ml) VASP peptide in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsVASP Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)
Tissue Location Highly expressed in platelets.
Research Areas
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$ 95.00
Cat# PBV10339b
(40 western blots)
Availability: 5-7days
Bulk Size
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