|Calculated MW||61002 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Nectin-3. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Nectin-3, CDw113, Poliovirus receptor-related protein 3, CD113, PVRL3, PRR3|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Nectin-3/CD113 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis- mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.|
|Cellular Location||Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane|
|Tissue Location||Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines|
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